Diagnosis can be made when mobile adult worms are visualized by a healthcare provider in the perianal region. Worms are best visualized 2 to 3 hours after the child is asleep.

Parents frequently mistake fecal debris or tissue paper for pinworms and unless the healthcare provider recognizes mobile adult worms, we recommend confirming parental diagnosis with stool or perianal “pinworm paddle” testing for microbiological confirmation of pinworm eggs.